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Furthermore, the expression of candidate miRNAs was validated in serum samples from an independent cohort of 186 individuals with CRC, 60 postoperatively, 43 with advanced adenomas and 53 controls. Subsequently, the authors analyzed miR-21 expression in Nu-lotan CRC tissue samples to determine whether serum miRNAs reflected its expression in CRC. Survival analysis was buy acichek online with the Kaplan-Meier method and Cox regression models.