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Researchers from Johns Hopkins University analyzed different cell clones from primary tumors of several patients which included the original population of cells that had given rise to the tumor and others that gave way to the different metastases, as well as samples of metastasis that occurred in the same individuals, to subsequently evaluate epigenetic parameters. The authors performed large-scale reprogramming of chromatin modifications during the natural history of metastasis. The changes were targeted to thousands of large chromatin domains across the genome that collectively specify malignant traits.