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This study investigated whether HDAC activity is altered in patients with RA and in a mouse model of house dust mite HDM-induced allergic asthma and whether it contributes to HDAC dysfunction. epithelial barrier. To address this issue, primary xolegel epithelial cells from control subjects and RA patients were cultured to study transepithelial electrical resistance and paracellular flux of fluorescent hexane isothiocyanate 4 kDa along with mRNA expression and immunofluorescence staining of tight junctions. Cultures were stimulated with different concentrations of JNJ-26481585, a broad-spectrum HDAC inhibitor. Finally, the effect of the inhibitor on mucosal permeability and tight junction function was evaluated in vivo in a mouse model for the study of HDM-induced allergic airway inflammation.
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